Transferability of apple and pear SSRs to other temperate pome fruit crops of family Rosaceae

Extensive use of simple sequence repeat (SSR) is facilitated if loci would be transferable across species even in closely related genera to overcome high cost and efforts involved their development as major constraints. In the present study, apple pear genomic microsatellite primer pairs were used amplify SSR apple, pear, quince loquat genotypes, respectively. Already reported SSRs selected based on polymorphic survey for successful amplification with at least one polymerase chain reaction (PCR) product approximate size expected a homologous locus screened among genotypes further transferability exploration other temperate pome fruit crops, Highest SSR, 61.53 % 73.33 was observed This indicated that binding sites between these two genera, Malus Pyrus, are fairly well conserved. Maximum rate found 93.33 80.00 all subjected CH05D11 TSUenh016 The markers similarity, can reflect relationship genome collinearity evolution species. level crops promise application future molecular screening, map construction, comparative studies, etc.